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RANKL에 의한 파골세포 분화에 Superfect 와 Tfx의 효과에 관한 연구
Effects of transfection reagents Superfect and Tfx on the RANKL-induced differentiation of RAW cells into osteoclast-like cells

구강생물학연구 2002년 26권 1호 p.149 ~ 159

이홍찬, 상현숙, 김흥희,

소속 상세정보

이홍찬 (  ) - 조선대학교 치과대학 미생물학
상현숙 (  ) - 조선대학교 치과대학 미생물학
김흥희 (  ) - 조선대학교 치과대학 미생물학

KMID : 0353320020260010149

Abstract

The ability to introduce DNA into mammalian cells provides a powerful means to study the function and control of mammalian genes. Common techniques for gene (DNA) transfer into eukaryotic cells include calcium phosphate. DEAE-Dextran, electroporation and more recently, liposome-mediated transfection. When reagents for liposome-mediated transfection were tested with RAW264.7 cells that can differentiate into osteoclast-like cells upon RANKL treatment. Superfect was found to reduce the differentiation whereas TfxTM had no effects. Therefore, TfxTM may be a more suitable reagent fot introducing DNA into cells in osteoclastogenesis studies.